Nitric oxide reductase(P450nor)from Fusarium oxysporum. A self-sufficient cytochrome P450 with a primary structural organization that includes a flavin domain and aredox center. Rational and semi-rational engineering of cytochrome P450s for biotechnological applications. Practical application of cytochrome P450. Cytochrome P450 systems-biological variations of electron transport chains. Evolution of bioinorganic motifs in P450-containing systems. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. Mechanism of oxidation reactions catalyzed by cytochrome p450 enzymes. Cytochrome P450 compound I: capture, characterization, and C-H bond activation kinetics. Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. How similar are P450s and what can their differences teach us?. Cytochrome P450 monooxygenases: perspectives for synthetic application. Biochim Biophys Acta Protein Struct Mol Enzymol, 2000, 1543(2): 383-407. Protein engineering of cytochromes P-450. Cytochrome P450 diversity in the tree of life. ![]() The carbon monoxide-binding pigment of liver microsomes. P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. ![]() Key words: cytochromes P450, flavor and fragrance, biosynthesis, protein engineering, metabolic engineering Finally, the challenges and prospects of their practical applications are discussed. The biosynthesis of several natural flavors and fragrances such as limonene oxygenated derivatives, nootkatone, santalol, 4-hydroxyisophorone, and macrocyclic musk, which are catalyzed by single enzyme-catalyzed biotransformation or whole-cell de novo biosynthesis involving protein and metabolic engineering, are reviewed. Hereby the mechanism of selective hydroxylation of P450s and related several common types of electron transfer chain system are introduced. Among the P450 catalyzed reactions, the introduction of a hydroxyl group at a specific inert C-H bonds under mild conditions makes them highly useful in high value-added fine chemicals biosynthesis. P450- catalyzed reactions demonstrate various advantages including wide substrate spectrum, diverse types of catalytic reactions, and high catalytic regio-and stereospecificity, which make them increasingly attractive in drug/toxin metabolism, and engineered product biosynthesis. ![]() P450s are known as “golden catalysts”, and they are mainly involved in the synthesis of endogenous substances, metabolism of xenobiotics, and biosynthesis of natural products in living organisms. Cytochromes P450 belong to a large superfamily of hemeproteins, which are widespread in almost all forms of life.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |